Cytology
毛醫師
Exfoliative
Uterine
Endometrial
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Washing
brushing
Aspiration
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病理部三種
Pap smear
Cell block
------------
Transformation
zone
HPV
Columnar
metaplasia to squamous
10顆良好的才是好片子
8000-12000顆細胞
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Liquid base
5000顆
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固定液95%酒精15-30min
Cytospin
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Cell
block. For pleura
10% formalin
Architecture
Special stain
for tumor unknown origin
Eg Thyroid
transcription factor 1 nuclear stain
Follicular
cell, parafollicular cell
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On site
cytology
Air dry cell
inflation
Inclusion
比較清楚
Papanicolaou
stain
Hematoxylin
nuclear
OG squamous orange
Light green
cyanophilic NC ratio high cell
Eosin Y
eosinophinic epithelium
------------
LSIl CIN 1
HSIL CIN 2/3
Low risk,
mainly HPV 16
High risk ,
AIS. HPV 16 mainly
------------
現在glandular type 越來越多
------------
Pap smear
台灣一年，而國外三年
HPV test +
Pap smear 五年一次
Corposcope
------------
------------
Koilocyte
Irregular
nuclear
Multiple
nucleus
Coarse
chromatin
Irregular
cell membrane thickness
------------
------------
Squamous
Intralepithilial lesion
------------
P53 E6
PRB E7 E2F
P16 staining cytoplasm
and nuclear
Ki67 nuclear
------------
Liquid based
prepararion
固定是用酒精
Remove mucus
and blood
Further IHC
p16, Ki67 double stain
Molecular
studies
- 20 mm Thinprep
- SurePath, density sedimentation 13mm
------------
Bethesda
system 2014
Organism
Candida
Trichomonas
vaginalis
Endometrial
cell >45 years
Out phase
>10 days after menstruations
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