[醫如往常] Senior Clerkship Day 106: Pathology: markers

孫法醫師

Frozen

O.C.T:optimal cutting temperature compound -19--21度

Molecule Liquid Nitrogen -85度

------------

Special stain

Congo red: Amyloid

Copper: Wilsons' disease

PAS; Periodic acic Schiff stain: glycogen, MPS, capsule, fungus

DPAS; diastase : salivary

Silver: hepatoma
Orcein: elastic fiber, aneurysm, AVM

Von Kossa 照光: Calcium

Warthin-Starry: spirochette

PTAH(Phosphotungstic 鎢 acid haematoxylin): striation (rhabdomyosarcoma), fibrin (DIC)

Fresh tissue:

Fat stain

Thio-acetylcholine esterase (AchE): megacolon, lamina propria

------------

IHC

CD30: Anaplastic large cell lymphoma (ALCL)

classical Hodgkin Lymphoma Reed-Sternberg cells

CD45: CLA; common leukocyte antigen

CD68: macrophage

CD117(c-kit): GIST

plasmacytoma lambda常見

CK7: UGI

CK20: LGI

Ki-67: proliferation

P63: breast myoepithelial cells

GFAP: glioma

ALK: Lung

P40: SCC

MSH6: MSI; microsatellite instability

TTF-1: Thyroid, lung adenocarcinoma

Synaptophysin, Chromogranin: Neuroendocrine peptides

------------

Positive, negative control

------------

白色 biopsy

黃 大件檢體

綠色 切兩片

藍色 牙科

粉紅色 autopsy

------------

10% neutral formalin (4% formaldehyde) 10x tissue量 + phosphatate buffer

滲透 1mm/ 1 hr

極限:五小時 上下加起來1cm

------------

Pancreas autolysis, fat necrosis

------------

Alcohol dehydration

Large cell -> small cell

------------

兩小時內

Bouin's solution-> bone marrow, spermatozoa

Masson's trichrome refixation

------------

Carnoy's solution -> EBV -> NPC

Acetic acid 冰

------------

吳法醫師

CD 79a pan B cells

CD 20 mature B cells

CD10 follicle cells

CD23 dendritic cells,  marginal type small BCL

BCL-2 apoptosis

------------

Myocarditis治療

Hypothermia

ECMO Bypass