IHC Markers
鄭醫師
------------
Pleomorphic undifferentiated sarcoma(abbreviated PUS), also undifferentiated
pleomorphic sarcoma and previously malignant fibrous
histiocytoma(abbreviated MFH), is a type of soft tissue sarcoma.
PUS occurs most commonly
in the extremities and retroperitoneum, but has been reported in other sites. Metastasisoccurs
most frequently in the lungs (90%), bones (8%), and liver(1%).
------------
IHC
Peroxidase
Purple
DAB+H2O2->O2+H2O+brown DAB
AEC紅色
Fab+Fc<2nd
Ab< enzyme
avidin biotin complex 許世明副院長
Endogenous
biotin background interference
Fab+Fc<2nd
Ab-PPP 只要三小時
一次抽血
Mouse 200 ul
Rabbit 50 ml
------------
Counterstain
Blue
hematoxylin
------------
Melanoma
S-100
(paraganglion也有)
HMG45
------------
Glioma(Astro,
Ependymomas, GBM)
GFAP; glial
fibrillary actin protein
Oligodendroma
FISH:
1p19q 自費一萬元
------------
Hematology
T CD3
B CD20
NK CD56
Macrophage
CD68
Myeloid MPO
Hodgkin CD30
------------
Sarcoma
Rhabdomyosarcoma,
embryonal: mRNA myogenin
Leiomyosarcoma:
desmin
Angiosarcoma CD31
Liposarcoma
lipoblast
Amplification of MDM2, CDK4
GIST
C-kit
Dog-1
Synovial
sarcoma
CK, TLE-1
MPNST
s-100(-),
fibroblast H3K27Me3
不用染IHC的:
Osteosarcoma
osteoid
Chondrosarcoma
cartilage
------------
Carcinoma
Squamous cell
carcinoma
P40
(p63,
squamous, basal 都有)
Cervix HPV
抑制p105RB, 表現p16
------------
Adenocarcinoma
Prostate: PSA
Lung: TTF-1 (Routine 已經變成用分子定義了)
ALK1
Breast: ER,
PR, Her2, GATA3
Liver: HepPar 1 (直接到liver cells到老鼠做出來的antibody, antigen不明) sensitivity 80%
Arginase 1: sensitivity
90%
Stomach,
Colon: CDX-2 (mRNA)
Gyn/GU: Pax-8 (mRNA)
Pancreas,
gall bladder: morphology
Stomach: annexin A10 for signet
ring cells
------------
Molecule
EGFR
KRAS
------------
IHC
Herpes
CMV
NTUH沒有進:
Adenovirus
Helicobacter
pylori
------------
Collision tumor
gastrointestinal epithelial malignant tumor (EMT)
epithelialmesenchymal
transition (EMT)
------------
沒有留言:
張貼留言
歡迎指教