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2016年12月4日 星期日

[醫如往常] Senior Clerkship Day 93: Lab Medicine: 藍紅黃綠紫灰黑

20161123
李醫師
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Good Marker
  • Sensitivity
  • Specific
  • Low molecular weight
  • Long half life
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Syncope
VT
  • >120 /min
  • 1/3
  • AV dissociation
  • Capture beat
  • Fusion beat

V1 rbbb
Lbbb
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Pacemaker
  • Subclavian vein
  • LBBB
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ANB monocyte
CAE eosinophil
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Cytospin
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AML
Cd34 immature
Cd11b, 15 mature
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A Coulter counter [1] [2] is an apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles.[3]
A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions. As fluid containing particles or cells is drawn through each microchannel, each particle causes a brief change to the electrical resistance of the liquid. The counter detects these changes in electrical resistance.
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CML
Basophil
Eosinophil

Megakaryocytes small
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藍紅黃綠紫灰黑
  • Blue 3.2% citrate (以前用3.6%): PT, aPTT 
  • 採血量較少時,依1:9比例使用。
  • : 生化
  • 綠色 heparin: NH3, LDH
  • Purple EDTA: CBC, Hb 電泳, HbA1C
  • Gray NaF EDTA: glucose
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ACD 淺黃 lymphocytes
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助教授
血小板生命 兩周
全血 四個月

Fy-ab (-) anti-malaria效果,非洲人很多
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MCHC 正常值33+-3 代表RBC central pale zone的比例
RDWCV 正常值13+-1.5
Ret 正常<2%
IRF(Immature Retic Fraction)=(HFR+MFR)/Reticulocyte 正常<20%
------------
遇到Platelet EDTA agglutination
改用citrate

MDS plt沒有granule會不準確
------------
NTUH每天6000個CBC 常規35項
1200要做WBC differential
300機器會自動染色觀看
60個需要人工染色

NTUH是大宗的
所以NHI打七折給付


Positive: 異常
  • count
  • differential
  • morphology
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Chédiak–Higashi syndrome[1] is a rare autosomal recessive disorder that arises from a mutation of a lysosomal trafficking regulator protein,[2] which leads to a decrease in phagocytosis. The decrease in phagocytosis results in recurrent pyogenic infections, partial albinism and peripheral neuropathy. It occurs in humans, cattle, blue Persian cats, Australian blue rats,[3] mice,[4] mink,[4] foxes,[4] and the only known captive albino orca.[5]


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[醫如往常] Senior Clerkship Day 92: Lab Medicine: Hepatology Clinical Chemistry Instrument

Clinical Chemistry Instrument
醫師
Spectrophotometry
------------
Chemistry
Tungsten 350-800nm
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Endogenous interference

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Flow cytometry
Laser
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Monochromator

Diffraction gratings

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Cuvets
Sample holder
Quartz, plastics, borosilicated glass
乾淨的水
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Detector
Photodiode array

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Beer's law


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Indicator
Chromophores
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Glucose hexokinase method
NADNADH

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化學標準化
1U = Catalyze 1 umole substrate in 1 minute
37℃

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Immunoassay 沒有標準化
因為各家抗體不一樣

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High dose hook effect
Lattice precipitation

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Pancreas cancer cyst change
Pancreatitis

幾十萬測出來只有幾千

先dilute 再做就會準了
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Turbidimetry
可以直接在生化儀上做
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Light scatter
Nephelometry


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太大太小的東西不能測


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Labeled method
  • Radioactive 準確度高
  • Fluorescence
  • Luminescence 藍色為主, 干擾少
  • Enzyme 便宜, 干擾多
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競爭性的是雙曲線, 因此只有中間準

非競爭型正關係比較準

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Heterogeneous assay
ELISA
  • 大小分子都可以驗
  • 敏感性高
  • 不過相對不精密
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化學冷凝光

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Evening 洪醫師
異常值一小時 危急值半小時


移植八項= 針扎十項-AST, ALT
  • Anti-HBVs
  • HBV Surface antigen
  • Anti-HBVcore
  • Anti-HCV
  • HIV ag/ab
  • Syphilis VDRL/RPR,  FTA-ABS/ TP-PA

前兩項HBV不會緊急通報
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CSF五項antigen
  1. Streptococcus pneumoniae
  2. Streptococcus group B ?
  3. HiB; Haemophilus influenzae type B
  4. Mycobacterium tuberculosis
  5. Escherichia coli (carrying the K1 antigen).
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流感快篩
  • -
  • A
  • B
  • A+B (可能是干擾)
  • 無法判定
共五種結果
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[醫如往常] Senior Clerkship Day 91: Lab Medicine: Diagnostic Hepatology

Hepatology 醫師

data異常一定要去看病人
Hyperglycemia-> 可能是從D5W IV側抽血造成的
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AST
  • 80% mitochondria
  • Half-life 17 hrs
  • Acute >10x

ALT
  • Chronic
  • Half-life 47 hrs
  • Acute >10x
    • Viral (>2000IU is very dangerous)
      • Jaundice 70% HAV(good prognosis), 35%HBV, 25%HCV
      • Mild fever通常是HAV,糞便要用lysol消毒,vaccination兩劑撐廿年,incubation 1mo,症狀出現之前糞便就有病毒
      • HBV分八種genotype,華人常見BC, C outcome很差,不過genotypebiofunction沒有甚麼實際差異。疫苗要>10IU/ml才有保護力。Anti-Hbe spontaneous seroconversion 10-20% annually. Adult得的<5% carrier

HBsAg
HBsAb
HBeAg
HBcAb
HBV DNA
Acute
+
-
+
IgM
+
Clerared infection
-
+
-
IgG
-
vaccinated
-
+
-
-
-
Inactivated carrier
+
-
-
IgG
<2000IU/ml
Chronic infection with active replication
+
-
+/-
IgG
>2000IU/ml
  • HCV 80%會變成carrier, asymptomatic,
  • HEV: High risk for pregnancy->fulminant hepatic failure. Window period 6-8 weeks.
  • Ischemia
  • Toxin/drug (3000IU是正常的,排除underlyingOK) Ex: statin (2.5% population)
  • Autoimmune
    • Type 1
      • ANA (Cytoplasmic); anti nuclear antibody
      • ASMA; anti smooth muscle antibody
      • AAA; anti actin antibody
    • Type 2
      • ALKM1; anti liver kidney microsomal I antibody
      • ALCM; anti liver cytosol 1 antibody
Ref.周貝倫 自體免疫性肝炎 - Taiwan Society of Internal Medicine http://www.tsim.org.tw/journal/jour20-3/03.PDF

  • Early phase of obstruction (jaundice & pain)
    • PBC; Primary Biliary Cirrhosis: AMA
    • PSC; Primary Sclerosing Cholangitis
    • Gallstones, CBD stones, pancreatic cancers
  • AST/ALT 3-5X
    • NASH; non-alcoholic steatohepatitis
      • 不一定fat有關,可能跟biofilm有關
      • 可能變成HCC
      • Asymptomatic
  • Gilbert syndrome
    • 2-10% population
    • Unconjugated bilirubin levels fluctuate
    • No risk of kernicterus to the fetus (不用浪費錢驗bilirubin)
    • R/o hemolysis
  • Wilson's disease
    • Low Ceruloplasmin (homozygous 65-95%, heterozygous 20%)

Factors
  • BMI
  • Exercise: Independent AST
  • Hemolysis: AST ALT Red urine, Occult blood, pale face, dizziness
  • Rhabdomyolysis: AST ALT Red urine, Occult blood(-), pale face(-)
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Gamma GT
  • Confirm ALP due to hepatic source
  • Alcohol
  • Drugs: statin
  • BMI
  • steatohepatitis (一般視為正常人)

ALP
  • Liver and bone
  • BMI, pregnancy
  • Marker of cholestasis
  • Half-life 1 week
  • 可以用ALP isoenzyme (很貴), 5-NT, gamma GT 驗證

Conjugation Bilirubin
  • Exercise
  • Hemolysis
  • Hemolytic anemia

INR

Thrombocytopenia
  • Liver fibrosis

Albumin
  • Half-life 19-21 days
  • Nephrotic syndrome
  • Burns
  • Enteropathy
  • SIRS
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ALP
AST
ALT
 GGT
 T-bil
TP
Albumin
Hepatocellular pattern
-
-



Cholestatic pattern
-
-



Mixed pattern



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對於無症狀的人,做預防醫學
Anti-Hbs IgG, anti-HCV, Anti-HAV IgG
------------

對於 RUQ pain, jaundice, fever, AST/ALT>1000 U/L
Anti-Hbc IgM, HbsAg, Anti-HAV IgM, anti-HCV (rare acute onset)

[醫如往常] Senior Clerkship Day 91: Lab Medicine: Anemia and RBC

林醫師 Anemia and RBC
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HTLV; human T-lymphotropic virus: ATLL; Adult T-Cell Leukemia/Lymphoma
------------
Needle shot 傳染率
HBV ~30%
HCV ~3%
HIV ~0.3%

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Myeloid
  • Megakaryocytes
  • Erythrocyte
  • Neutrophil
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  1. Proerythroblast
  2. Erythroblast (basophilic->Polychromatic->Orthochromatic)
  3. Normoblast(Orthochromatic)(NRBC) (Nucleated RBC): 正常周邊血不會有
  4. Reticulocyte

Erythrocyte七天成熟, 壽命 120天
Erythropoietin: 90% kidney, 10% liver
Heme = Fe+ protoporphyrin
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History taking
  • Exertional dyspnea
  • Dizziness
  • Fatigue

Etiology
  • Age, gender
  • Blood loss: MC, hemorrhoids, GI
  • Diet, vegetarians, B12
  • Operation, gastrectomy
  • Thalassemia history α4% β2%
  • Blood data
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Colon cancer, stool OB
------------
  • B12; cobalamin: ileum
  • B9
  • Fe: duodenum
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Thalassemia
  • Β homozygous severe
  • α3 intermediate. HbH disease
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Lab data
  • Hb, MCV, RBC
  • RDW 紅血球變異度
  • Reticulocyte
  • PB smear
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RPI
Hematocrit (%) Retic survival (days) = maturation correction
36-45 1.0
26-35 1.5
16-25 2.0
15 and below 2.5
RPI<0.1%
  • PRCA
  • AA
RPI>3%
  • Bleeding,
  • hemolytic anemia
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MCV
>115
  • Megaloblastic
  • Chemotherapy
  • MDS

100-115
  • Hypothyroidism
  • Alcoholism
  • Ditto
  • Reticulocytosis
  • Bone marrow

80-100
  • ACD chronic disease
  • Renal failure
  • Mixrf deficiency
  • Bone marrow diz

<80
  • IDA
  • Thalassemia
  • Sideroblastic anemia
  • Pb intoxication
  • ACD
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Small lymphocytes 8-9 um
RBC 7um
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IDA
  • Microcytic hypochromic
  • Central pale zone 正常應該1/3-1/4
  • Anisocytosis 大小不一
  • Poikilocytosis 形狀
------------
  • MCV
  • RBC
  • High RDW
  • High MCV/RBC. (DDx thalassemia RBC normal)
  • Mild plt

  • Ferritin low (Normal 20~300)
  • TIBC high
  • Iron/TIBC <16% (normal 33%)

(Ddx ACD HIGH ferritin, normal TIBC)


  • Koilonychia
  • Glossitis
  • Angular celitis
------------

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Thalassemia
  • Target cell
  • Poikilocytosis
  • Hemolysis
  • Normoblast

  • HbA2>3.5%
  • HbF>2%
------------
250cc blood transfusion 100mg Fe
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Megaloblastic anemia
  • MCV
  • Hypersegmented neutrophils.   (MDS 也會)
  • Pancytopenia
  • Hemolysis
  • Tinnitus, neurological S/S
  • Vegetarians
  • Gastrectomy
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Pernicious anemia, antibody to IF Intrinsic factor
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"台大醫院新竹分院血液腫瘤科醫師林耘曲表示,經抽血後發現,溫婦的白血球、血色素、血小板都偏低,進一步檢查才發現,溫婦是罹患典型的維生素B12缺乏症,一開始會出現食慾不振、體重減輕,甚至開始出現記憶力衰退等情形,長期下來恐導致骨髓衰竭及早年失智,所幸在幫溫婦注射維生素B12針劑後,溫婦情況大為好轉,脾氣也變好了。"
Normal 270~400
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Pancytopenia
P: PNH
A: Asplatic anemia
N: Neoplasm/ Near neoplasm (including MDS)
C: Cirrhosis/ Connective tissue disease
Y: Vit B12/ Folic acid
T: Toxin/ Drug
O: Overwhelming sepsis (Hemophagocytic
syndrome)/ Others

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Hemolytic anemia
  • Reticulocytosis
  • Indirect hyperbilirubinemia
  • Increased LDH, very sensitive. (Lymphoma也敏感)
  • Decreased haptoglobin (結合free form hemoglobin)
  • Erythroid hyperplasia in marrow
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必考填空題
Vascular Hemolysis
Haptoglobin<30
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Extravascular Hemolysis: spleen
------------
Approach
Immune
  • AIHA; autoimmune
  • Drug-induced
Non-immune
  • Microangiopathic TTP (Adamts 13 切割vWF), MAHA
  • HUS
  • DIC
  • mechanical prosthetic heart valve
  • Hypersplenism
  • PNH
  • Infection
  • Congenital: G6PD, thalassemia, spherocytosis


------------
TTP
  • LDH >1000
  • Thrombocytopenia不能輸Platelets (有爭議)
  • Plasmapheresis
  • NTUH 一年2-3例
  • Helmet cells

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Coombs' test
  • Direct: RBC, 較有臨床意義, Anti- C3b,IgG
  • Indirect: serum
------------
Cold AIHA
  • IgM pentamer, cold agglutination
  • Mycoplasma infection
  • Lymphoma
  • CAD

以前有個流浪漢寒流就送急診,肺癌

Warm
  • MicroSpherocyte
  • IgG帶到Spleen (extracellular)
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Comparison

  • Multiple myeloma
    • Linear agglutination (Rouleaux formation)