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2016年9月30日 星期五

[醫如往常] Senior Clerkship Day 18: Ob/Gyn SSCT; Urogynecology

    Grand Round PGY
    SSCT; Spermatogonial stem cell autotransplantqtion
    ------------
    Subfertility (prevalence: 15%)
    Subfertility generally describes any form of reduced fertility with prolonged time of unwanted non-conception.

    • 17% male factor
    • Drug, cancer
    ------------
    Testis biopsy→Cryo-sperm→Propagation
    Rete testis, efferent ductules injection
    ------------
    Crisper/Cas9 gene editing
    Clustered regularly interspaced short palindromic repeats
    NGG, NCC PAM(protospacer adjacent motif)
    CRISPR/Cas (Clustered regularly interspaced short palindromic repeats and CRISPR-associated systems) 是將來自於化膿性鏈球菌(Streptococcus pyogenes)的Cas9蛋白與guide RNA形成一個複合體,由guide RNA上的Protospacer序列(17~20nt)辨識DNA,緊接在Protospacer序列之後的三個核苷酸(NGG)稱為Protospacer adjacent motif(PAM),Cas9即辨識PAM並切割緊鄰的雙股DNA。其後又發展出只切割單股DNA的CAS9 Nickase以降低脫靶效應(off target effect)。
    Protospacer adjacent motif (PAM) is a 2-6 base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system.
    ------------
    GeCKO; Genome scale CRISPR-Cas9 knockout
    Lentivirus
    ------------
    41% azoospermia 仍可TESE; Testicular sperm extraction
    ------------

    Urogynecology 蕭醫師

    Level I
    Cardinal lig.

    Level II, cystocele
    Arcus tendineus (fasciae pelvis),white line

    Level III
    Perineum
    ------------
    Radical hystectomy
    C1 nerve-sparring
    C2 non-nerve-sparring
    ------------
    Sup hypogastric n.: Sympathetic
    Inf. Hypogastric plexus: mixed
    ------------
    Obturator n.
    Adductor
    ------------
    POP-Q system
    D: omit when no cervix
    Valsalva maneuver才準
    ------------
    -3,-3,-8,-10,-3,-3,11,4,3
    n Aa, Ba, C, D, Ap, Bp, total vaginal length,
    genital hiatus, perineal body


    Simplified POP-Q
    n Ba
    n Bp
    n C
    n D
    ------------
    SUI
    Sphincter defect
    Urethral hypermobility. Q Tip test
    1. Resting angle>30°
    ------------
    Functional capacity >300 cc
    ------------
    20 min pad test
    ------------
    Voiding cystometry
    Detrusor pressure=bladder-abd
    Normal 10-25 mmH2O
    ------------
    UPP
    Maximal urethral closure pressure

    SUPP
    壓力傳導能力
    Urethral hypermobility
    ------------
    Correlation of LUTD before and after UDS

    ------------
    VLPP(Valsalva Leak Point Pressure)<60
    200 cc leak pressure
    ------------
    Pharmacologic Treatment of SUI
    n Duloxetine : serotonin-norepinephrine reuptake inhibitor (SNRI)
    n Imipramine : Anticholinergic agents and α-adrenergic agonist
    ------------
    MMK: cause Pelvic bone osteolysis
    ------------
    TVT for ISD, Intrinsic sphincter defect
    ------------
    先排除UTI
    OAB

    Solifenacin antimuscarinic for 3 months
    Mirabegron beta-3 agonist

    Sacral neuromodulation
    Botox
    ------------
    Uterus prolapse
    Venous stasis may be related

    Ip Vaginal sacrospinous colpopexy
    ------------

[醫如往常] Senior Clerkship Day 20: Ob/Gyn 婦科常識

超音波
黃醫師 TMU教學長

Uterus往膀胱: 前傾
TAS 1000cc & wait 2 hour.(~500cc in bladder)
TVS 不會看到膀胱,在右下角
------------
Uterus 7cm
Postmenopausal 3-5cm
------------
Fetal head size uterus
Enlargement
------------
18mm follicle near ovulation
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Uterine myoma
Progesterone antagonist: Mifepristone
自費,一天兩百元
可縮小一半
------------
單極電刀用蒸餾水
雙極D5 water
------------
Uterine Sarcoma 3-6‰
Ddx
  • Lung metastasis
  • Urachal cancer
  • Colon cancer

------------
Adenomyosis
Abdominal mass
Dysmenorrhea
Peak age 40
Menometrorrhagia. Duration 10 days, irregular, 非月經期也流血

Treatment
Danazol. 長鬍子雄性化聲音變低不可逆
停經
黃體素避孕器Mirena副作用點狀出血
Subtotal resection, 不要wedge, 三年復發, 懷孕破裂
------------
備血 最快半小時
Colloid
------------
驗孕
沒尿就驗血
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止血可用Oxytocin
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Endometrial cancer
Spotting bleeding
Menometrorrhagia

Risk
PCOS

Lymph node Meta
Stage 3c1, 2, 3

IA         Tumor confined to the uterus, no or < ½ myometrial invasion
IB         Tumor confined to the uterus, > ½ myometrial invasion
II          Cervical stromal invasion, but not beyond uterus
IIIA      Tumor invades serosa or adnexa
IIIB      Vaginal and/or parametrial involvement
IIIC1    Pelvic node involvement
IIIC2    Para-aortic involvement
IVA       Tumor invasion bladder and/or bowel mucosa
IVB       Distant metastases including abdominal metastases and/or inguinal lymph nodes

想懷孕high dose progesterone三個月
只有stage 1A IVF

一定要拿掉卵巢

停經後出血10% cancer
衛生紙擦到棕色
------------
Substitute Pregnancy
US$200,000
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Abortion in TW要先生同意書
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避孕器在腹腔:要拿, 怕腸子破掉
Common infection
Actinomycosis
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Polyp亮亮
月經後出血

Risk
Tamoxifen
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Low segment CS scar
超音波像小火龍

小心之後Ectopic Pregnancy
Acreta
Ercreta

不建議VBAC; vaginal birth after Cesarean

Myomectomy過的一定CS
------------
OHSS
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PID
CBC
TOA
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月經中來診
  • Luteal cyst rupture 性行為,被撞到
  • Ectopic Pregnancy
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Sand-like Chocolate cyst
  • 不會torsion
  • 經痛

Surgery indication
  • Infertility
  • Too big would rupture
  • Symptom
------------
Huge pelvis cyst
Bladder retention
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Thin septum <0.3 cm
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Sausage-like Fallopian
Hydrosalpinx
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Tubal plasty 成功率很低
直接做IVF
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五週超音波才看得到
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Grand round次專科討論會
RPL; recurrent Pregnancy loss
50% unknown
50% aneuploidy
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PGS降低流產率
36%>13% miscarriage
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Idiopathic miscarriage
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Day 5 > day3 準確
Array CGH >FISH 準確
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PGS
US$45300
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>37 yo PGS比較cost effective
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周醫師
Abdominal girth 腹圍增加
Palpable mass >10cm
Pregnancy 20 weeks-> umbilicus
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超音波 脂肪 黑色
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Mucinous cystadenoma
Multicystic, septum<4 mm
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少一顆卵巢,提早2-5年更年期
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手術注意
  • Bilateral
  • Check metastasis
  • Colon
  • Appendix
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Pseudoperitoneum, PP
Malnutrition
Die
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家族史: 提早十年開始篩檢乳癌
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吳醫師 禾馨副院長
Colposcopy
1924 Hinselmann

biopsy交界
Advanced study: 醋酸 蛋白變性 Pathology
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Pap smear 70% 檢測率
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Syphilis
Primary one dose
Secondary 3 weeks
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Genital warts
Wartec 8 weeks
Aldara 12 weeks
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Trichomoniasis
Metronidazole 一次八顆 0.25 g
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Phthirus pubis 陰蝨
除毛
曬太陽
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Monilia
Candidiasis
Fluconazole one dose
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Chronic vulvar inflammation
Lichen sclerosis
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PID 診斷要件
Sex within 6 month
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[醫如往常] Senior Clerkship Day 24: Ob/Gyn 產科手術; Partogram

產科手術 (黃醫師)
Forceps >600 kinds, (Fetal-medicine Committee, ACOG; American College of Obstetricians and Gynaecologists鑒定)
Chamberlain 17th century 最早
4 parts: Blade, shank, lock, handle
Cephalic curve, pelvic curve

English lock: Simpson (鏤空), Tucker-Mc Lane (solid) forceps
Sliding lock: Kielland

Indication: Elective outlet forceps (DeLee 1920)
常見併發症neonatal jaundice
------------
Prerequisites for forceps delivery
  1. Emptying of bladder
  1. Engagement
  2. Vertex presentation or MA in face presentation. (MP need Cesarean)
    1. the vertex presentation: the occiput is the leading part
    2. MA: Mento-Anterior
  3. Position
  4. Full dilatation of cervix (effacement)
  5. ROM; rupture of membrane
  6. No CPD
------------
Local anesthesia: pudendal nerve
Epidural anesthesia (EA): Meperidine
------------
VED; Vacuum Extraction Delivery
1840 Simpson
放在small fontanelle,不要在large fontanelle, frontal
0-100 cm-Hg, 一般用50-60.
1954  Malmstrom, artificial caput (chignon=bun)
Complications:
  1. Scalp laceration
  2. Subgaleal hematoma
  3. Cephalohematoma: periosteum, not cross midline
  4. ICH
------------
Breech delivery: 3-4% of singleton
  • Full Breech: frank: spontaneous dropout
  • Incomplete Breech: footling: pop out
Mainly preterm baby
Elective Cesarean
原則:不介入、晚介入
常用
Piper forcep
Complications: fractures
------------
Version
Week 32 以上的胎位才有意義
Internal podalic version: 抓腳,不要抓手,避免paralyzed arms
------------
Vernix Caseosa至少要等出生後的6-24小時處理才會比較妥當。
------------
CD; Cesarean Delivery(不要寫C/S, C-S,不專業)
德文Kaiserschnitt; 拉丁文Caedere 切開的意思, 本身就是切開的意思,所以不用再加上section
Lapratomy+  hysterotomy
(abdominal pregnancy, uterine rupture只有lapratomy不能叫做CD)
Taiwan ~40%
美帝 ~32.9%
台大總院 ~30%

Indication:
  1. Previous CD (Repeat CD)
  2. Dystocia- CPD; Cephalopelvic Disproportion
  3. Malpresentation 胎位不正
  4. Fetal distress
  5. CDMR (Cesarean delivery on maternal request) 婦女權美帝保險有給付
  6. Maternal Eisenmenger syndrome, RV failure (NTUH ECMO生過)
  7. Previous uterine surgery
  8. Preeclampsia
  9. Major bleeding
  10. Poliomyelitis, lame induced non circle bony pelvis (骨盆不對稱或變形)
  11. Short stature < 150 cm
  12. Hydrocephalus
  13. Meningocele (超音波發現就直接CD,避免浪費時間)
  14. Soft Parts Dystocia,  including the vulva, perineum, vagina, cervix, uterus, adnexa, rectum and bladder.

NPO for 8 hours
  • EA or GA
  • SA,血壓掉打麻黃素
  • Ketamine intern, 加上muscle suppression
  • Local (以前appemdititis也是打local)

現在主流low segment transverse uterine incision with abdominal longitudinal incision
1912 Kronig 1926 Kerr 發明
Low segment血管較少(主要是uterine a. 橫的分支)
Upper segment 很多肌肉,血管很多
Pfannenstiel bikini cut 很費時
Vertical uterine incision (1912Kronig)可能會往下裂開,很難trace

現在也會放一些tissue barrier,減少adhesion
現在縫內兩層,外層自然adhesion,減少手術時間
1882 Max Sanger提出要縫合

轉為傳統術式的indication:
  • Low segment adhesion with bladder, myoma, invasive cervical carcinoma
  • Large fetus with transverse lie (too big to take out)
  • Placenta previa with anterior implantation
  • Very small fetus in breech

1916 Cragin, "Once a Cesarean, always a Cesarean." (classical vertical)
1978 UTMerrill & Gibbs提倡VBAC; vaginal birth after Cesarean (transverse)
成功率不高
  • 1980 17%
  • 1988 25%
  • 2007 8.5%
VBAC risk: 0.1% Uterine rupture (1999 Leveno)
------------
古代沒有abx,會做extraperitoneal CD (ECS)
  • 1907 Frank
  • 1909 Latzko
  • 1940 Waters
減少peritonitis
但是很容易adhesion
------------
Cesarean hysterectomy (1876 Porro's OP.)(CD + subtotal hysrectomy)
Indication:
  1. Intrauterine infection
  2. POP; Pelvic Organ Prolapse
  3. Uterine scar
  4. Hypotonic uterus with PPH; Postpartum Hemorrhage 院外轉來很多,可以先給radiologistUAE看看
  5. Major vessel laceration
  6. Large myoma
  7. Cervix cancer
  8. Placenta creta (accreta, incerta, or percreta)
------------
PMR; Perinatal mortality rate(No. of death under age 1) / (1000 live births)
NMR; neonatal = (No. of deaths under 4 wks & >GA 28 weeks) / (1000 live births)
 Neonatal death = Deaths under 4 wks (28 days, roughly 1 mo.)
評估醫療水準
C.f. Parity >GA 20 weeks
------------
自然分娩及接生案例討論  (江醫師) 
1.藍單 2.產程圖(cervical curve跟胎位),胎兒心音圖及子宮收縮圖
------------
NSD; Normal Spontaneous Delivery
------------
3P 必要條件
  • Power
  • Passenger
  • Passage
  • ------------
SO-B line
Suboccipital-Bregma 9.8cm
Deflexion:
  • SO-F (frontal) 11cm
  • SO-M (mentol) 12.5cm
------------
產程圖(Partogram)(超重要)
Labor分幾個stage? 
  • Stage1:開始真正產痛到子宮頸全開,又分latent phase平均8.6hrs(cervix 0~3cm),active phase平均4.9hrs (acceleration(3~4cm)、phase of maximum slope(4~9cm)、deceleration(9~10cm))如圖所示。
  • Stage2:子宮頸全開到胎兒娩出,會有bear down sensation(便意感),一般在初產婦為50minutes,經產婦為20 minutes。
  • Stage3:胎兒娩出至胎盤娩出,約半小時以內。
  • Stage4:胎盤娩出後一小時內。
------------
Latency phase (8hr), <1cm/hr
Active phase (2hr), >1cm/hr
Acceleration
Maximum (通常達到7-8cm,進入deceleration)
deceleration
------------
  • Inter-ischial line 0 cm
------------
Engagement
Station 0 cm
Cervical dilatation ~7-8cm
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Full
  • Cervical dilatation 10 cm
  • Station +2 cm
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初產婦
Latency 最多等20hr
Active phase最多各等3hr
所以first stage最多等29hr
Second stage最多等2hr
------------
prolonged latent phase
  • primipara: True labor後>20hrs未進入active phase
  • multipara: True labor後>14hrs未進入active phase
充足水份與休息即可,Oxytocin*加強宮縮緊急狀況應 CS
------------
Protraction  disorder  (產程遲滯)
觀察、休息 如有 CPD 則 C/S
protracted active phase dilatation—最常見
  • primipara: 子宮頸擴張<1.2cm/hr 
  • multipara: 子宮頸擴張<1.5cm/hr
protracted descent
  • primipara: 子宮頸全開後,胎頭下降 <1cm/hr
  • multipara: 子宮頸全開後,胎頭下降 <2cm/hr
------------
下降停滯(Arrest of descent) station < 0cm
  • primipara: 在active phase,胎頭>1hr沒有下降
  • multipara: 在active phase,胎頭>1hr沒有下降

胎頭無法下降(Failure of descent) station < 2cm
  • primipara: 在deceleration phase或stage2胎頭沒下降
  • multipara: 在deceleration phase或stage2胎頭沒下降
------------
Med86 林思宏醫師 OBGYN Clerkship & Internship Survival Guide Version 4.0 p114
------------
Modified Bishop score
Cervical length replace effacement
  • 0 for >3 cm,
  • 1 for >2 cm,
  • 2 for >1 cm,
  • 3 for >0 cm.

Bishop Score (依照判讀順序)
  1. Station
  2. Effacement
  3. Position
  4. Consistency
  5. Dilatation


Parameter
Score



Description

0
1
2
3

Fetal station
3
2
1, 0
+1, +2
Fetal station describes the position of the fetus' head in relation to the distance from the ischial spines, which are approximately 3-4 centimetres inside the vagina and are not usually felt. Health professionals visualise where these spines are and use them as a reference point. Negative numbers indicate that the head is further inside than the ischial spines and positive numbers show that the head is below the level of the ischial spines.
0-30%
40-50%
60-70%
80+%
Effacement translates to how 'thin' the cervix is. The cervix is normally approximately three centimetres long, as it prepares for labour and labour continues the cervix will efface till it is 'fully effaced' (paper thin).
Position
Posterior
Middle
Anterior
The position of the cervix changes with menstrual cycles and also tends to become more anterior (nearer the opening of the vagina) as labour becomes closer.
Consistency
Firm
Medium
Soft
In primigravid women the cervix is typically tougher and resistant to stretching, much like a balloon that has not been previously inflated (it feels like the bottom of a chin). With subsequent vaginal deliveries the cervix becomes less rigid and allows for easier dilation at term.
Closed
12 cm
34 cm
5+cm
Dilation is a measure of how open the cervical os is (the hole). It is usually the most important indicator of progression through the first stage of labour.

2016年9月29日 星期四

[醫如往常] Senior Clerkship Day 16: Ob/Gyn Cases

戴總醫師
Gelform 38000
Endocatch 8000
------------
45歲以前拿子宮有傷殘補助沿用35年的勞保生育失能給付(包括切除子宮),將有重大修正。鑑於各界質疑女性切除子宮訂定45歲給付門檻是歧視女性,勞動部正規畫以大幅放寬條件、但分級給付金額的方式處理;也就是取消女性切除子宮年齡限制,但分三級給付,其中男性切除陰莖、女性切除子宮,給付標準由160天的投保薪資降為100天。
勞保失能給付中所指的「喪失生育能力」,女性係指包括「切除子宮」、「切除兩側卵巢」及「因為化療或放療致喪失生育能力」。自68年以來,由於考慮女性大都45歲以後就不生育,因此以45歲做為給付門檻,女性45歲以下切除子宮者才可請領給付,但此舉引發各方爭議。婦科醫師更發現很多女性為了經濟因素,搶在45歲之前切除子宮,造成台灣45歲以下「無子宮」切除率太高;但婦女團體則認為,男性因生殖器官切除喪失生育能力,並無年齡限制,獨對女性設限,明顯歧視。
勞保局統計,自97年以來,失能給付案每年有2.9萬至3.3萬件,其中「喪失生育能力」案件從97年7萬多件降到101年的6萬多件,件數逐年降低,但比率都在20%-22% 。
勞保局統計,目前每年3萬多件失能給付個案中,2成(6700件)是生育失能給付,政府支付近9億元,除了個位數的男性切除睪丸之外,絕大多數都是女性切除子宮、卵巢。 來自 <http://health.udn.com/health/story/5999/474610>
------------
鄭教授必考
Round lig.Major labium
The round ligament of the uterus originates at the uterine horns, in the parametrium. The round ligament enters the pelvis via the deep inguinal ring,[2] passes through the inguinal canal and continues on to the labia majora[3] where its fibers spread and mix with the tissue of the mons pubis.
The round ligament is supplied by the artery of the round ligament, otherwise known as "Sampson's artery."
------------
Afternoon meeting
PGD
Epidermolytic ichthyosis (EI); Epidermolytic hyperkeratosis (EHK)
(also known as Bullous congenital ichthyosiform erythroderma(CIE),[1] Bullous ichthyosiform erythroderma,[2]:482 or bullous congenital ichthyosiform erythroderma Brocq[3])來自 <https://en.wikipedia.org/wiki/Epidermolytic_hyperkeratosis>

AD
keratin 1 (KRT1) and keratin 10 (KRT10)
Incidence  1 in 200,000-300,000
紅孩兒穿山甲
Some patients may experience amelioration of symptoms as they age. Risk for morbidity and mortality is highest in the neonatal period, where infants are at increased risk for complications such as sepsis and dehydration because of impaired barrier function. Later in life, affected patients may experience recurrent skin infections.
------------
Mosaic tuner syndrome 1/3000

Low grade 45xo 身高正常

Only 2-10% spontaneous conceiving and they are mosaic.
Only 5.7% live birth.

Amniocentesis mosaic, normal
可能拿到的是placental cell
------------
Irregular menstrual cycle
Bleeding

Left lower limb swelling and pain
DVT
EkoS catheter; The EkoSonic™ Endovascular System

Endometrial adenocarcinoma

Trousseau syndrome
May be double cancer
The Trousseau sign of malignancy or Trousseau's Syndrome is a medical sign involving episodes of vessel inflammation due to blood clot (thrombophlebitis) which are recurrent or appearing in different locations over time (thrombophlebitis migrans or migratory thrombophlebitis). The location of the clot is tender and the clot can be felt as a nodule under the skin.[1] Trousseau's syndrome is a rare variant of venous thromboembolism (VTE) that is characterized by recurrent, migratory thrombosis in superficial veins and in uncommon sites, such as the chest wall and arms. This syndrome is particularly associated with pancreatic and lung cancer.[2]
Trousseau's Syndrome can be an early sign of gastric or pancreatic cancer,[3] typically appearing months to years before the tumor would be otherwise detected.[4] Heparin therapy is recommended to prevent future clots.[5] The Trousseau sign of malignancy should not be confused with the Trousseau sign of latent tetany caused by hypocalcemia.
------------
Discordant MCDA twins
------------
Ritscher-Schinzel cranio-cerebello-cardiac (3C) syndrome
3C syndrome, also known as CCC dysplasia, Craniocerebellocardiac dysplasia[1] or Ritscher–Schinzel syndrome,is a rare condition, whose symptoms include heart defects, cerebellar hypoplasia, and cranial dysmorphism. It was first described in the medical literature in 1987 by Ritscher and Schinzel, for whom the disorder is sometimes named.
3C syndrome is an autosomal recessive disease, caused by a mutation on the long arm of chromosome 8 at 8q24.13, the locus for KIAA0196,
------------
Cleft palate
------------
Multicystic tumor at right neck
Cystic hygroma, lymphatic malformation

Risk
  • Aneuploidy
  • Trisomy 21
  • Turner syndrome
  • Noonan syndrome
  • Fryns syndrome
  • Multiple pterygium syndrome
  • Fetal alcohol syndrome
  • Cardiac and skeletal

Poor outcome
  • Nuchal thickness > 6 mm
  • Karyotype anomaly
  • Ultrasonography anomaly

Ddx
  • Increased nuchal translucency
  • Neural tube defect
  • Cystic teratoma
  • Hemangioma

Medication not good
Surgery May recur
------------
IUFD
Cord accident
Week 22
------------
ER
UTI
------------
ROV tumor

RLQ tenderness
Immature teratoma
Chemotherapy相當有效
也可能是良性的,只是因為懷孕影響morphology

Ddx
Dysgerminoma
------------
Uterine inversion
Atony
Vaginal laceration
Gauze packing
------------
Uterine carcinosarcoma
------------